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Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
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Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Article DOI: 10.3791/2593-v • 07:14 min • April 26th, 2011
April 26th, 2011 •
1, 2, 3, 2, 3
1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 2Department of Molecular and Cell Biology, University of Connecticut, 3Department of Pharmaceutical Sciences, University of Connecticut

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Summary
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Nucleosome ELISA (NU-ELISA) is a sensitive and quantitative method to detect global patterns of post-translational modifications in preparations of native, intact nucleosomes. These modifications include methylations, acetylations, and phosphorylations at specific histone amino acid residues, and hence NU-ELISA provides a global proteomic assay of the overall chromatin modification states of specific cell types.

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Post-translational Modifications Native Intact Nucleosomes ELISA Chromatin Nucleosome Histones N-terminal Tails Acetylation Methylation PTMs Epigenetic Gene Regulation Nucleosome ELISA (NU-ELISA) Global PTM Signatures Epiproteomic State
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