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JoVE Journal
Neuroscience

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Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
 
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Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

Article DOI: 10.3791/2594-v 07:50 min April 6th, 2011
April 6th, 2011

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Summary

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Click here for the English version.

The present video demonstrates a method which takes advantage of the combination of electroporation and confocal microscopy to perform live imaging on individual neural progenitor cells in the developing zebrafish forebrain. In vivo analysis of the development of forebrain neural progenitor cells at a clonal level can be achieved in this way.

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Time-lapse Live Imaging Clonally Related Neural Progenitor Cells Developing Zebrafish Forebrain Brain Development In Vivo Environment Electroporation Fluorescent Markers Genetic Labeling Low Melting Point Agarose Glass Bottom Culture Dishes Confocal Microscope Water Dipping Objective Lens Central Nervous System Zebrafish Embryo
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