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Journal / Neuroscience / Balb / C 뇌 조직에 높은 민감도는 5 hydroxymethylcytosine…
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Neuroscience

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JoVE Journal Neuroscience
Balb / C 뇌 조직에 높은 민감도는 5 hydroxymethylcytosine 감지
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Balb / C 뇌 조직에 높은 민감도는 5 hydroxymethylcytosine 감지

Article DOI: 10.3791/2661-v • 07:20 min • February 1st, 2011
February 1st, 2011 •
1, 1
1Applications and Product Development, New England Biolabs

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EpiMark 5 HMC 5 - MC 분석 키트는 분석하고 spe cific 로커스 이내 5 methylcytosine 5 - hydroxymethylcytosine을 quantitate하는 데 사용할 수 있습니다. 키트는 β - glucosyltransferase (T4 - BGT)를 이용한 효소 반응을 통해 5 - HMC의 수산기 그룹에 포도당의 추가에 의해 5 - HMC에서 5 - MC를 구별. 5 HMC는 CCGG의 맥락에서 발생하면,이 수정은 아닌 cleavable 사이트에 cleavable MspI 사이트를 변환합니다.

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신경 과학 제 48 EpiMark Epigenetics 5 hydroxymethylcytosine 5 methylcytosine methylation hydroxymethylation
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