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Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
 
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Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Article DOI: 10.3791/2662-v 06:29 min March 1st, 2011
March 1st, 2011

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Summary

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Click here for the English version.

We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.

Tags

Pro-inflammatory Cytokine IL-1β Whole Blood Stimulation Assay Inflammatory Processes Immune Cells Cytokine Homeostasis Innate Immune System Pathogens Danger Stimuli Interleukin-1 (IL-1) Secretion Autoinflammatory Syndromes LPS Cellular Immune Responses PAMPs Gram-negative Bacterial Endotoxin
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