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Three-dimensional Optical-resolution Photoacoustic Microscopy
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Three-dimensional Optical-resolution Photoacoustic Microscopy

Article DOI: 10.3791/2729-v • 08:32 min • May 3rd, 2011
May 3rd, 2011 •
1, 1, 1
1Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis

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Summary
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Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.

Tags

Optical-resolution Photoacoustic Microscopy Biomedical Technology Cellular Level Organelle Level In Vivo Three-dimensional (3-D) Single-/multi-photon Fluorescence Microscopy Optical Coherence Tomography (OCT) Optical Absorption Contrast Biological Tissues Physiological/pathological Information Biomedical Photoacoustics Diffraction Limit Lateral Resolution Optical Absorption Contrasts Extraordinary Sensitivity Fluorescence Microscopy Optical-scattering-based OCT Comprehensive Optical Properties Acoustic Waves
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