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JoVE Journal
Biology

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活细胞钙成像相结合的siRNA介导的基因沉默标识CA 2 +泄漏通道内质网膜和监管机制
 
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活细胞钙成像相结合的siRNA介导的基因沉默标识CA 2 +泄漏通道内质网膜和监管机制

Article DOI: 10.3791/2730-v 13:41 min July 7th, 2011
July 7th, 2011

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

内质网在蛋白质生物合成和钙稳态的关键作用。我们已经建立了一个实验系统,使我们能够解决的Ca2 +泄漏通道的作用,并表征其公认的监管机制。该系统涉及的siRNA介导的基因沉默和活细胞内Ca2 +成像。

Tags

细胞生物学,53期,细胞钙稳态,钙调蛋白,​​互补,内质网,ER钙泄漏,基因沉默,智商图案,突变分析,Sec61复杂
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