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Isolamento di Brain-infiltrazione Leucociti
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Isolamento di Brain-infiltrazione Leucociti

Article DOI: 10.3791/2747-v • 06:44 min • June 13th, 2011
June 13th, 2011 •
*1, *1
1Department of Neurology, Mayo Clinic College of Medicine
* These authors contributed equally

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Un metodo rapido per ottenere leucociti infiltrati dal cervello murino è descritto. Questo metodo utilizza una pendenza costante Percoll e gradiente discontinuo di Ficoll selezionare e purificare i leucociti arricchito strato. Leucociti isolati può quindi essere caratterizzata da misure di citometria a flusso.

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Neuroscienze Numero 52 leucociti cervello mouse neuroimmunologia Theiler è murino encefalomielite virus citometria a flusso
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