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Immunology and Infection

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JoVE Journal Immunology and Infection
El aislamiento de la infiltración de leucocitos cerebro-
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El aislamiento de la infiltración de leucocitos cerebro-

Article DOI: 10.3791/2747-v • 06:44 min • June 13th, 2011
June 13th, 2011 •
*1, *1
1Department of Neurology, Mayo Clinic College of Medicine
* These authors contributed equally

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Un método rápido para obtener la infiltración de leucocitos en el cerebro murino se describe. Este método utiliza un gradiente de Percoll continua y discontinua gradiente de Ficoll para seleccionar y purificar la capa de leucocitos enriquecido. Leucocitos aislados se puede caracterizar por las mediciones de citometría de flujo.

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Neurociencia Número 52 leucocitos el cerebro el ratón neuroinmunología el virus de la encefalomielitis murina de Theiler citometría de flujo
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