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Journal / Immunology and Infection / Hücre olgunlaşması olmadan Dendritik Hücreler…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Hücre olgunlaşması olmadan Dendritik Hücreler Verimli Transfeksiyon için optimize edilmiş Protokolü
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
Journal (Immunology and Infection)
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Journal (Immunology and Infection)
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
 
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Hücre olgunlaşması olmadan Dendritik Hücreler Verimli Transfeksiyon için optimize edilmiş Protokolü

Article DOI: 10.3791/2766-v • 08:08 min • July 8th, 2011
July 8th, 2011 •
*1, *1, 1, 1
1Center for Translational Systems Biology and Department of Neurology, Mount Sinai School of Medicine
* These authors contributed equally

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Biz plazmid DNA hücresinin olgunlaşması neden olmaksızın veya siRNA birincil insan monosit türevli dendritik hücreler transfecting etkin bir yolu olarak optimize edilmiş yüksek verimli nucleofection protokol sunuyoruz. Biz daha başarılı bir şekilde hedef gen, mRNA ve protein seviyeleri hem de Rig-I siRNA susturulması için kanıt sağlar.

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İmmünoloji Sayı 53 Dendritik hücreler nucleofection yüksek verimli siRNA interferon sinyali
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