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Journal / Immunology and Infection / Optimerade protokoll för effektiv Transfektion av …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Optimerade protokoll för effektiv Transfektion av dendritiska celler utan cellmognaden
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Optimerade protokoll för effektiv Transfektion av dendritiska celler utan cellmognaden

Article DOI: 10.3791/2766-v • 08:08 min • July 8th, 2011
July 8th, 2011 •
*1, *1, 1, 1
1Center for Translational Systems Biology and Department of Neurology, Mount Sinai School of Medicine
* These authors contributed equally

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Vi presenterar våra optimerade hög genomströmning nucleofection protokoll som ett effektivt sätt att transfecting primära humana monocyt-härledda dendritiska celler med antingen plasmid DNA eller siRNA utan att orsaka cellmognaden. Vi ger ytterligare bevis för framgångsrik siRNA tysta riktade genen Rig-i på både mRNA-och proteinnivåer.

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Immunologi 53 dendritiska celler nucleofection hög genomströmning siRNA interferon signalering
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