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Immunology and Infection

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JoVE Journal Immunology and Infection
Immuno-fluorescens-analys av Leptospiral Surface-exponerade Proteiner
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Immuno-fluorescens-analys av Leptospiral Surface-exponerade Proteiner

Article DOI: 10.3791/2805-v • 10:47 min • July 1st, 2011
July 1st, 2011 •
1,2, 3,4
1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

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En effektiv metod för att bedöma ytaktiva exponering leptospiral proteiner beskrivs. Metoden är särskilt utformade för att undvika störningar av den bräckliga yttre membranet av leptospiral celler. Denna teknik kräver anställning av flera negativa kontroller för att bedöma integriteten av yttre membran och specificitet antikroppsreaktion.

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Immunologi 53 molekylärbiologi Leptospira intakta celler yttre membran yt-exponerade proteiner yta immuno-fluorescens
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