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Kwantificering van eiwitten met behulp van immuno-peptide Verrijking gekoppeld aan massaspectrometrie
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Kwantificering van eiwitten met behulp van immuno-peptide Verrijking gekoppeld aan massaspectrometrie

Article DOI: 10.3791/2812-v • 06:09 min • July 31st, 2011
July 31st, 2011 •
*1, *1, 2, 3, 4, 5, 2, 3, 1
1Clinical Research Division, Fred Hutchinson Cancer Research Center - FHCRC, 2Department of Biochemistry and Microbiology, University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre, University of Victoria, 5Plasma Proteome Institute
* These authors contributed equally

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Stabiele Isotopen Normen en Capture door Anti-peptide antilichamen (SISCAPA) paren affiniteit verrijking van peptiden met stabiele isotoop verdunning massaspectrometrie (MRM-MS) voor kwantitatieve meting van peptiden te bieden als surrogaat voor hun respectievelijke eiwitten. Hier beschrijven we het protocol met behulp van magnetische deeltjes in een deels geautomatiseerde formaat.

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Moleculaire Biologie massaspectrometrie gerichte test peptide MRM SISCAPA eiwit kwantificatie
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