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JoVE Journal
Neuroscience

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量化的活动顺调控元件外植体电穿孔
 
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量化的活动顺调控元件外植体电穿孔

Article DOI: 10.3791/2821-v 07:39 min June 28th, 2011
June 28th, 2011

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Summary

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Click here for the English version.

本协议描述了一个简单而廉价的方式来量化的顺式调控元件的活动(即,增强/促销员)通过外植体电小鼠视网膜生活。 DNA的制备,视网膜剥离,电,视网膜外植体文化,和固定后的分析和定量描述。

Tags

神经科学,第52期,视网膜感光细胞,顺式调控元件,量化,电,启动子分析
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