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Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination

Article DOI: 10.3791/2823-v • 07:43 min • August 1st, 2011
August 1st, 2011 •
1,2, 2,3
1Neuroscience Graduate Program, Keck School of Medicine, University of Southern California, 2Zilkha Neurogenetic Institute, University of Southern California, 3Department of Cell and Neurobiology, Neuroscience Graduate Program, Keck School of Medicine, University of Southern California

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Summary
Automatic Translation

An in vivo method to test gene function in postnatal brain is described. Recombinant AAVs expressing Cre and/or a fluorescent protein are injected into neonatal mouse brain. Mosaic gene inactivation and sparse neuronal labeling are achieved, allowing rapid analysis of gene function in processes critical to neural circuit development.

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Mosaic Analysis Gene Function Postnatal Mouse Brain Development Virus-based Cre Recombination Normal Brain Function Embryonic Development Neuronal Pathways Postnatal Development Neural Circuits Neurological Disorders Psychiatric Disorders Autism Schizophrenia Prenatal Brain Developmental Processes Gene Deletion Mouse Models Floxed Alleles Transgenic Alleles Conditional Deletion Gene Function Analysis Virally-expressed Cre Recombinant Adeno-associated Viruses (rAAVs)
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