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JoVE Journal Neuroscience
Screening Assay für oxidativen Stress in einem Feline Astrocyte Cell Line, G355-5
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Screening Assay für oxidativen Stress in einem Feline Astrocyte Cell Line, G355-5

Article DOI: 10.3791/2841-v • 09:53 min • July 13th, 2011
July 13th, 2011 •
1, 1, 1, 2, 3, 2, 1,2
1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products

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Ein Screening-Verfahren zur oxidativen zellulären Umgebungen erkennen ist, um die Oxidation von CM-H2DCFDA messen. Sobald in einer Zelle, CM-H2DCFDA Änderungen von nicht-fluoreszierenden in einen fluoreszierenden Verbindung oxidiert. Diese Änderung in der Fluoreszenz wird mittels Durchflusszytometrie gemessen und gibt die Anzahl der Zellen in einer oxidativen Umgebung.

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Neuroscience Ausgabe 53 Astrozyten oxidativer Stress Durchflusszytometrie CM-H2DCFDA
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