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JoVE Journal
Biology

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Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
 

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration

Article DOI: 10.3791/2845-v 07:27 min August 29th, 2011
August 29th, 2011

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Summary

Acute kidney injury (AKI) in humans is a common clinical problem caused by damage to the epithelial cells that comprise kidney nephrons, and AKI is associated with high mortality rates of 50-70%1. Following epithelial cell destruction, nephrons have a limited ability to regenerate, though the mechanisms and limitations that guide this phenomenon remain poorly understood. In this video article, we describe our technique for targeted laser ablation of kidney nephron cells in the zebrafish embryo kidney, or pronephros. Our new method can be used to complement nephrotoxicity-induced models of AKI and gain a high-resolution understanding of the cell and molecular alterations that are associated with epithelial regeneration in the kidney nephron.

Tags

Laser Ablation Zebrafish Pronephros Renal Epithelial Regeneration Acute Kidney Injury (AKI) Renal Function Fluid And Electrolyte Homeostasis Clinical Therapies Mammalian Models Nephron Tubules Epithelial Cell Death Proximal Tubule Cells Dedifferentiation Proliferation Migration Renal Epithelial Regeneration Mechanisms Larval Zebrafish
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