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JoVE Journal
Immunology and Infection

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Flow Cytometry Analysis of Immune Cells Within Murine Aortas
 
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Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Article DOI: 10.3791/2848-v 15:16 min July 1st, 2011
July 1st, 2011

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Summary

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Click here for the English version.

This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.

Tags

Flow Cytometry Immune Cells Murine Aortas Atherosclerosis Chronic Inflammatory Process Plaques Foam Cells Immune Response Innate Arm Adaptive Arm Macrophages Dendritic Cells T Lymphocytes B Lymphocytes Aortic Adventitia Atherogenesis Quantitative Detection RT-PCR Immunohistochemical Methods Flow Cytometry Analysis
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