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Het meten van de kinetiek van mRNA transcriptie in Single levende cellen
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Het meten van de kinetiek van mRNA transcriptie in Single levende cellen

Article DOI: 10.3791/2898-v • 11:22 min • August 25th, 2011
August 25th, 2011 •
1, 1
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University

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RNA polymerase II transcriptionele kinetiek worden gemeten op specifieke genen in levende cellen. mRNA getranscribeerd van het gen van belang zijn fluorescent gelabeld en het gebruik van Fluorescence Recovery After Photobleaching (FRAP) de in vivo kinetiek van transcriptionele rek worden verkregen.

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Celbiologie mRNA transcriptie kern live-cell imaging cellulaire dynamiek FRAP
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