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Att mäta kinetiken av mRNA-transkription i enskilda levande celler
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Att mäta kinetiken av mRNA-transkription i enskilda levande celler

Article DOI: 10.3791/2898-v • 11:22 min • August 25th, 2011
August 25th, 2011 •
1, 1
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University

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RNA-polymeras II transkriptionell kinetik mäts på specifika gener i levande celler. mRNA transkriberas från genen av intresse är fluorescerande taggade och med hjälp av fluorescens återhämtning efter fotoblekning (FRAP) in vivo kinetiken av transkriptionell töjning erhålls.

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Cellbiologi mRNA transkription kärna live-cell imaging cellulär dynamik FRAP
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