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Agrobacterium-Mediated Virus-Induced Assay silenciamento gênico Em Cotton
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Agrobacterium-Mediated Virus-Induced Assay silenciamento gênico Em Cotton

Article DOI: 10.3791/2938-v • 10:18 min • August 20th, 2011
August 20th, 2011 •
1, 1, 2, 1
1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University

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Apresentamos o protocolo detalhado para Agrobacterium mediada induzida pelo vírus ensaio de silenciamento de genes (VIGS) em algodão. O vírus chocalho tabaco (TRV) derivados de vetores VIGS foram mobilizados para induzir silenciamento de RNA de algodão GrCLA1, Cloroplastos alterados um gene. O fenótipo albino causada pelo silenciamento GrCLA1 ​​foi observada na fase de mudas dentro de 2 semanas após a inoculação.

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Biologia Vegetal Edição 54 Agrobacterium algodão Genómica Funcional Virus-Induced silenciamento gênico
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