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Journal / Bioengineering / Organotypic Collagen I Assay: A Malleable…
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Bioengineering

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JoVE Journal Bioengineering
Organotypic Collagen I Assay
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Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

Article DOI: 10.3791/3089-v • 10:46 min • October 13th, 2011
October 13th, 2011 •
1, 1, 1, 1, 2, 2, 1
1The Beatson Institute for Cancer Research, University of Glasgow, 2Section of Dermatology, School of Medicine, University of Glasgow

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A method is described for the preparation of a 3-dimensional matrix consisting of collagen type I and primary human fibroblasts. This organotypic gel serves as a useful substrate to assess invasive cell migration because it mimics basic features of tissue stroma and is amenable to many forms of microscopy.

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Organotypic Collagen I Assay Cell Behavior 3-dimensional Context Cell Migration Biology Development Wound Healing Immune System Tumor Cells Glass Coverslips Light Microscopy Local Environment Elasticity Protein Composition Pore Size Two-dimensional Substrates Stromal Fibroblasts Immune Cells Invasion Of Cancer Cells Molecular Dynamics Drug Treatment,response To Drug Treatment In Vitro And In Vivo Comparison Tissue Culture Systems Cell Derived Matrix Matrigel Organotypic Culture Tissue Explants
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