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JoVE Journal
Immunology and Infection

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Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
 

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

Article DOI: 10.3791/3123-v 09:17 min July 28th, 2011
July 28th, 2011

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Summary

A method is described to individually select, manipulate, and image live pathogens using an optical trap coupled to a spinning disk microscope. The optical trap provides spatial and temporal control of organisms and places them adjacent to host cells. Fluorescence microscopy captures dynamic intercellular interactions with minimal perturbation to cells.

Tags

Optical Trap Host-pathogen Interactions Dynamic Live Cell Imaging Immune System Phagocytic Cell Microbe Intercellular Contact Events Phagocytosis Cell-cell Contact Optical Trapping Optical Tweezers Biological Research
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