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Journal / Immunology and Infection / Separação de Single-stranded DNA, Double-stranded …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Separação de Single-stranded DNA, Double-stranded DNA e RNA de uma Comunidade Ambiental Viral Usando Cromatografia hidroxiapatita
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Separação de Single-stranded DNA, Double-stranded DNA e RNA de uma Comunidade Ambiental Viral Usando Cromatografia hidroxiapatita

Article DOI: 10.3791/3146-v • 13:47 min • September 29th, 2011
September 29th, 2011 •
1, 2, 1
1Department of Microbial and Environmental Genomics, The J. Craig Venter Institute, 2Department of Synthetic Biology and Bioenergy, The J. Craig Venter Institute

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Nós descrevemos um método eficiente para separar single-stranded DNA, dupla fita de DNA e RNA de moléculas ambientais comunidades viral. Os ácidos nucleicos são fracionados por cromatografia em fase de hidroxiapatita com concentrações crescentes de fosfato contendo buffers. Este método permite o isolamento de todos os tipos de ácido nucléico viral a partir de amostras ambientais.

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Imunologia Edição 55 hidroxiapatita DNA de fita simples dupla fita de DNA RNA DNA cromatografia ecologia viral vírus bacteriófago
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