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Journal / Biology / Kwantificering en analyse van de vorming van…
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Biology

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JoVE Journal Biology
Kwantificering en analyse van de vorming van HO-endonuclease chromosomale translocaties Gestimuleerd door Single-Strand Gloeien in Saccharomyces cerevisiae
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Kwantificering en analyse van de vorming van HO-endonuclease chromosomale translocaties Gestimuleerd door Single-Strand Gloeien in Saccharomyces cerevisiae

Article DOI: 10.3791/3150-v • 09:41 min • September 23rd, 2011
September 23rd, 2011 •
1,2, 2, 3, 2
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology, City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology, University of Southern California, Norris Comprehensive Cancer Center

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De HO-gestimuleerde translocatie assay monitoren enkelstrengs gloeien na de oprichting van DNA dubbelstrengs breuken op meerdere loci in diploïde

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Genetica translocatie formatie HO-endonuclease Genomic Southern blot chromosoom blot Pulsed-field gel elektroforese homologe recombinatie DNA dubbelstrengs breuken Single-streng gloeien
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