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JoVE Journal
Immunology and Infection

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从核衣壳的病毒DNA的制备
 
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从核衣壳的病毒DNA的制备

Article DOI: 10.3791/3151-v 12:46 min August 16th, 2011
August 16th, 2011

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Summary

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Click here for the English version.

我们描述了从被感染的细胞中分离高纯度的疱疹病毒衣壳DNA的过程。从溶液中捕获的是最后的DNA浓度和纯度高,使得它非常适合高通量测序,高保真PCR反应,并产生新的病毒重组体转染。

Tags

免疫学杂志,54期,核衣壳的DNA病毒,单纯疱疹病毒(HSV)的,伪狂犬(PRV),测序
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