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Journal / Immunology and Infection / Beredning av viral DNA från Nucleocapsids
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Immunology and Infection

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JoVE Journal Immunology and Infection
Beredning av viral DNA från Nucleocapsids
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Beredning av viral DNA från Nucleocapsids

Article DOI: 10.3791/3151-v • 12:46 min • August 16th, 2011
August 16th, 2011 •
1, 1, 1
1Department of Molecular Biology, Princeton University

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Vi beskriver processen att isolera hög renhet herpesvirus nukleokapsid DNA från infekterade celler. Den slutliga DNA fångas från lösning med hög koncentration och renhet, vilket gör den idealisk för hög genomströmning sekvensering, high fidelity PCR reaktioner och transfections att producera nya virus rekombinanter.

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Immunologi 54 viral nukleokapsid DNA herpes simplex virus (HSV) pseudorabies (PRV) sekvensering
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