Here, we describe a protocol for the purification of highly active Hsp104, a hexameric AAA+ protein from yeast, which couples ATP hydrolysis to protein disaggregation. This scheme exploits a His6-tagged construct for affinity purification from E. coli followed by anion-exchange chromatography, His6-tag removal with TEV protease, and size-exclusion chromatography.
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Sweeny, E. A., DeSantis, M. E., Shorter, J. Purification of Hsp104, a Protein Disaggregase. J. Vis. Exp. (55), e3190, doi:10.3791/3190 (2011).
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Hsp104 is a hexameric AAA+ protein1 from yeast, which couples ATP hydrolysis to protein disaggregation2-10 (Fig. 1). This activity imparts two key selective advantages. First, renaturation of disordered aggregates by Hsp104 empowers yeast survival after various protein-misfolding stresses, including heat shock3,5,11,12. Second, remodeling of cross-beta amyloid fibrils by Hsp104 enables yeast to exploit myriad prions (infectious amyloids) as a reservoir of beneficial and heritable phenotypic variation13-22. Remarkably, Hsp104 directly remodels preamyloid oligomers and amyloid fibrils, including those comprised of the yeast prion proteins Sup35 and Ure223-30. This amyloid-remodeling functionality is a specialized facet of yeast Hsp104. The E. coli orthologue, ClpB, fails to remodel preamyloid oligomers or amyloid fibrils26,31,32.
Hsp104 orthologues are found in all kingdoms of life except, perplexingly, animals. Indeed, whether animal cells possess any enzymatic system that couples protein disaggregation to renaturation (rather than degradation) remains unknown33-35. Thus, we and others have proposed that Hsp104 might be developed as a therapeutic agent for various neurodegenerative diseases connected with the misfolding of specific proteins into toxic preamyloid oligomers and amyloid fibrils4,7,23,36-38. There are no treatments that directly target the aggregated species associated with these diseases. Yet, Hsp104 dissolves toxic oligomers and amyloid fibrils composed of alpha-synuclein, which are connected with Parkinson's Disease23 as well as amyloid forms of PrP39. Importantly, Hsp104 reduces protein aggregation and ameliorates neurodegeneration in rodent models of Parkinson's Disease23 and Huntington's disease38. Ideally, to optimize therapy and minimize side effects, Hsp104 would be engineered and potentiated to selectively remodel specific aggregates central to the disease in question4,7. However, the limited structural and mechanistic understanding of how Hsp104 disaggregates such a diverse repertoire of aggregated structures and unrelated proteins frustrates these endeavors30,40-42.
To understand the structure and mechanism of Hsp104, it is essential to study the pure protein and reconstitute its disaggregase activity with minimal components. Hsp104 is a 102kDa protein with a pI of ~5.3, which hexamerizes in the presence of ADP or ATP, or at high protein concentrations in the absence of nucleotide43-46. Here, we describe an optimized protocol for the purification of highly active, stable Hsp104 from E. coli. The use of E. coli allows simplified large-scale production and our method can be performed quickly and reliably for numerous Hsp104 variants. Our protocol increases Hsp104 purity and simplifies His6-tag removal compared to a previous purification method from E. coli47. Moreover, our protocol is more facile and convenient than two more recent protocols26,48.
1. Expression of Hsp104
- The plasmid employed for purification in E. coli, pPROEX-HTb-Hsp104, contains the Hsp104 open-reading frame under the inducible control of the trc promoter26. The plasmid produces Hsp104 with an N-terminal His6-tag that can be removed by TEV protease cleavage. Transform pPROEX-HTb-Hsp104 into codon-optimized E. coli BL21-CodonPlus-RIL cells (Stratagene, Agilent Technologies) using a typical bacterial transformation procedure (e.g. according to manufacturer's instructions). It is important to use a codon-optimized E. coli strain because Hsp104 has an unusual codon bias.
- Inoculate a 100mL 2XYT culture (USB. Recipe: 16g/L casein peptone, 10g/L yeast extract, 5g/L NaCl, pH 7.0) supplemented with 100μg/mL ampicillin and 34μg/mL chloramphenicol with fresh transformants and grow overnight at 37°C with shaking at 200rpm.
- Inoculate 10mL of the overnight culture into 6 X 1L 2XYT with 100μg/mL ampicillin and 34μg/mL chloramphenicol in 2L flasks. Shake at 250rpm at 37°C until OD600 = 0.4-0.6. Stop shaking and reduce temperature to 15°C. Allow cells to equilibrate unagitated for 30min in the incubator until it reaches 15°C. Once 15°C is reached induce protein expression by adding IPTG to a final concentration of 1mM. Resume shaking at 250rpm overnight (12-16 hours).
2. Cell Harvest and Lysis
- Harvest cells by centrifugation (in a precooled rotor) at 4,000rpm for 20min at 4°C (we use a Sorvall RC 3BP+ centrifuge). Subsequent steps must be performed immediately because Hsp104 activity is diminished when E. coli cells are frozen. Moreover, all subsequent steps should be performed on ice or at 4°C.
- Resuspend cell pellets in prechilled (on ice) 10mL lysis buffer (40mM HEPES-KOH pH 7.4, 500mM KCl, 20mM MgCl2, 2.5% (w/v) glycerol, 20mM imidazole, 5μM pepstatin A, complete protease inhibitor cocktail (1 EDTA-free tablet/50mL), and 2mM β-mercaptoethanol).
- Lyse cells with a French press (Emulsiflex) homogenizer that uses a heat exchanger immersed in ice water. Before use, ensure that all cell clumps have been solubilized. After equilibrating homogenizer with lysis buffer, two passes through the cylinder with a pressure of 15,000-18,000psi is sufficient for full lysis. If a French press is not available, incubation with lysozyme followed by sonication can be used (see discussion). Save a small sample of lysed cells for SDS-PAGE analysis (1μL) (Lysate in Fig. 2).
- Remove cell debris by centrifugation at 16,000rpm for 20min at 4°C (we use a Sorvall RC5C+ centifuge). Retain supernatant for next step and save a small sample (1μL) of supernatant for SDS-PAGE analysis (Ni Load in Fig. 2).
3. Hsp104 Purification
- Mix supernatant from step 2.4 with 12mL (2mL Ni-Sepharose beads per 1L of cells) of 50% slurry of lysis buffer equilibrated Ni-Sepharose beads (GE).
- Rotate sample slowly for 3 hours at 4°C such that Ni-Sepharose remains evenly suspended and frothing is minimized. Shorter incubation times are possible, but will decrease yield. At 4°C in the presence of protease inhibitors, little degradation occurs, and a 3 hour Ni-Sepharose incubation time does not decrease activity of the final product. Collect Ni-Sepharose by centrifugation for 2 min at 4°C at 2,000rpm (Eppendorf 5810R centrifuge). Save a small sample of supernatant for SDS-PAGE analysis (1μL) and discard the rest (Ni Flow Through (FT) in Fig. 2).
- Wash the retrieved Ni-Sepharose with 25 column volumes of wash buffer (40mM HEPES-KOH pH 7.4, 150mM KCl, 20mM MgCl2, 2.5% (w/v) glycerol, 20mM imidazole, 2mM β-mercaptoethanol), 5 column volumes of wash buffer with 1M KCl to remove contaminants bound electrostatically to Hsp104, and 25 column volumes of wash buffer. Collect Ni-Sepharose after each wash by centrifugation for 2 min at 4°C at 2,000rpm (Eppendorf 5810R centrifuge). After each wash and centrifugation cycle, remove buffer by aspiration.
- To elute Hsp104, mix Ni-Sepharose with 1mL elution buffer (40mM HEPES-KOH pH 7.4, 150mM KCl, 20mM MgCl2, 2.5% (w/v) glycerol, 350mM imidazole, 2mM β-mercaptoethanol) per 1mL Ni-Sepharose and rotate at 4°C for 20 minutes. The high imidazole concentration disrupts the Ni bead-His6 interaction. Remove Ni-Sepharose with empty 1.2 mL spin columns (Bio-Rad) by centrifugation for 2 min at 4°C at 2,000rpm (Eppendorf 5810R centrifuge). Save 1μL of eluate for SDS-PAGE analysis (Ni eluate in Fig. 2). For every liter of cells, ˜15mg of Hsp104 is obtained at this step.
- Buffer exchange eluate into Buffer Q (20mM Tris-HCl pH 8, 0.5mM EDTA, 5mM MgCl2, 50mM NaCl) using MWCO 30,000 Amicon Ultra (Millipore) 15mL centrifugal concentrator units. First, concentrate protein to ˜1.5mL then add 14.5mL of Buffer Q to dilute protein 10-fold. Repeat 3 times for buffer exchange. The increased pH and low salt ensures that Hsp104 has a high negative charge.
- Purify Hsp104 via anion-exchange chromatography using a Buffer Q equilibrated Resource Q 6mL column (GE). Before injection, protein is filtered through a low protein binding Millex GP PES Membrane 0.22μm syringe filter (Millipore). We employ a flow rate of 1mL/min. Wash away weakly binding proteins with 1 column volume of 20% Buffer Q+ (20mM Tris-HCl pH 8, 0.5mM EDTA, 5mM MgCl2, 1M NaCl). Elute Hsp104 with linear gradient (20%-50% Buffer Q+) over 5 column volumes (Fig. 3). Collect 1ml fractions. Hsp104 and most variants typically elute at ˜400mM NaCl (31mS/cm). Save samples of peak fractions (1μL) for SDS-PAGE analysis (Fig. 3, inset).
- To remove His6-tag, exchange protein using the Amicon centrifugal concentrator as describe above (see step 3.5) into cleavage buffer (20mM HEPES-KOH pH 7.4, 140mM KCl, and 10mM MgCl2). Use proTEV protease (Promega) or AcTEV protease (Invitrogen) according to manufacturer's instructions. For Hsp104, we have found that higher ratios of TEV protease: Hsp104 are required for full cleavage. We use a ratio of 1 protease unit per 12μg Hsp104. Cleavage should be performed at 30°C for 2-4 hours, followed by a 16 hour incubation at 4°C. Final Hsp104 concentration should be between 20-75μM monomer. Deplete any remaining His6-tagged Hsp104 and TEV protease with Ni-Sepharose (GE) by adding Ni-Sepharose in excess to the amount of Hsp104 in the cleavage reaction (assume beads can bind 15mg of protein per mL of packed resin). Remove beads with empty spin columns (Bio-Rad) by centrifuging for 2min at 2,000rpm at 4°C. Collect samples before and after cleavage (1μL) for SDS-PAGE analysis to ensure cleavage, and the removal of uncleaved protein (Fig. 4).
- Exchange into size exclusion buffer (20mM HEPES-KOH pH 7.4, 140mM KCl, 10mM MgCl2, 0.1mM EDTA, 1mM DTT) using MWCO 30,000 Amicon Ultra (Millipore) as described in step 3.5.
- Further purify Hsp104 via size-exclusion chromatography. Use size exclusion buffer equilibrated Superose 6 or Superdex 200 column (both from GE) (Fig. 5). Before injection, protein is filtered through a low protein binding Millex GP PES Membrane 0.22μm syringe filter (Millipore). For less than 10mg of protein, the 10/300 size columns (24mL) can be used and 0.5ml fractions are collected. For samples greater than 10mg, the 12/60 size column (120mL) should be used and 1 ml fractions are collected. Refer to manufacturer's instructions for flow-rates and sample volumes to be loaded. After purification, Hsp104 fractions are pooled as shown in Fig. 5 and concentrated in Amicon Centrifugal Concentrator Units (Millipore) [Editor's Note: Video voice over incorrectly states 0.22μM (micromolar) syringe filter, when it should state 0.22μm (micrometer) syringe filter at timecode 04:38.]. Hsp104 is stored as described below. Due to loss of material in separating Hsp104 degradation products and contaminants the final yield of purified full-length Hsp104 is ˜1-3mg per liter of starting culture.
4. Hsp104 Disaggregase Activity
- After purification, it is good practice to assess Hsp104 activity in a disaggregation assay. Typically, we employ a luciferase reactivation assay2 (Fig. 6). In this assay, Hsp104 in conjunction with the Hsp70 chaperone system (Hsp70 and Hsp40) disaggregates urea-denatured firefly luciferase aggregates2. Soluble luciferase catalyzes the oxidation of luciferin to oxyluciferin, a reaction that releases light. By monitoring luminescence, the relative reactivation, and therefore disaggregation, of luciferase can be determined. Hsp104 must be able to synergistically collaborate with the Hsp70 co-chaperone system. The amount of recovered luminescence depends on the exact Hsp70:Hsp40 pair utilized. We routinely employ Hsp72 and Hsp40 (Assay Designs). At a minimum, active Hsp104 should produce a 5-fold increase in reactivation of luciferase when compared to reactivation by Hsp72:Hsp40 alone (Fig. 6). Hsp104 preparations that fail to reach this level of activity are discarded.
5. Hsp104 Storage
- For short-term storage, Hsp104 can be kept at 4°C in size-exclusion buffer. However, activity will decline after 2-3 days at 4°C and sharply after 1 week at 4°C. For disaggregation assays we recommend that Hsp104 should be used as soon as possible after purification. Ideally, Hsp104 should be used immediately for amyloid disaggregation assays.
- If protein must be stored long-term, Hsp104 is exchanged into storage buffer (20mM HEPES-KOH pH 7.4, 140mM KCl, 10mM MgCl2, 0.1mM EDTA, 1mM DTT, 10% glycerol (w/v)). 100μl aliquots are snap frozen in liquid nitrogen and stored at -80°C. Freeze-thaw cycles drastically reduce Hsp104 activity and should be avoided. We recommend slowly thawing Hsp104 on ice. Amyloid-disaggregase activity will decline sharply after 1 month at -80°C.
6. Representative Results and Figures:
Figure 1. Hsp104 is a Bifunctional disaggregase. Disaggregation of disordered aggregates (shown on left) requires the cooperation of the Hsp70 chaperone system (Hsp70 and Hsp40)2. Hsp104 remodels ordered amyloid aggregates (shown on right) without the aid of Hsp70 and Hsp40 in vitro, but Hsp70 and Hsp40 can improve Hsp104 activity against amyloid26,28. For both types of aggregated structures, Hsp104 couples ATP hydrolysis to substrate translocation through its central channel to promote disaggregation. Tyrosine-bearing pore loops engage and shuttle substrate through the central channel49-52.
Figure 2. SDS-PAGE analysis of Ni-sepharose affinity purification step. Lysate, Ni Load, Ni FT and Ni eluate samples were fractionated by SDS-PAGE using a 4-20% Tris-HCl 1.0mm Criterion gel (Bio-Rad) and Coomassie stained. Note that not all the Hsp104 is able to bind to the Ni-sepharose. Broad Range molecular weight markers (Bio-Rad) are shown (left lane).
Figure 3. Resource Q purification of Ni-sepharose eluate. Blue trace represents absorbance at 280nm and green trace represents % Buffer Q+ (maximum at 50%). The first peak, which elutes at 20% Q+ contains impurities, degradation products and improperly folded Hsp104. The second and major peak contains properly folded and active Hsp104. Flow rate was 1ml/min. Gradient from 20-50% Q+ is 30 minutes or 5 column volumes. Inset: peak fractions are resolved by SDS-PAGE analysis using a 4-20% Tris-HCl 1.0mm Criterion gel (Bio-Rad) and Coomassie stained. Broad Range molecular weight markers (Bio-Rad) are shown (left).
Figure 4. SDS-PAGE analysis of proTEV protease cleavage step. His6-Hsp104 from Resource Q purification was treated with proTEV protease for 4 hours at 30°C and then 16 hours at 4°C. Samples were the fractionated by SDS-PAGE using a 4-20% Tris-HCl 1.0mm Criterion gel (Bio-Rad) and Coomassie stained. Note that proTEV cleaved Hsp104 migrates more rapidly. TEV protease and uncleaved Hsp104 have been depleted with Ni-Sepharose as described in Step 3.7. Broad Range molecular weight markers (Bio-Rad) are shown (left).
Figure 5. Size-exclusion purification of Hsp104. Cleaved Hsp104 was further purified via a Superose 6 gel filtration column (10/300, GE). Flow rate = 0.4ml/min. The peak between the dashed lines represents pooled fractions. Inset: peak fractions are resolved by SDS-PAGE analysis using a 4-20% Tris-HCl 1.0mm Criterion gel (Bio-Rad) and Coomassie stained. Broad Range molecular weight markers (Bio-Rad) are shown (left).
Figure 6. Luciferase reactivation assay. Denatured firefly luciferase aggregates (50nM) were incubated with either Hsp72 and Hsp40 (1μM) (both from Assay Designs), Hsp104 (6μM monomer) or Hsp104, Hsp72 and Hsp40 for 90min at 25°C. Denatured luciferase is only fully reactivated in the presence of both Hsp104 and Hsp40/Hsp72. Recovered luminescence is measured on an Infinite M1000 plate reader (Tecan). Values represent means±SEM (n=3).
Timeline: For maximal Hsp104 activity we recommend that the entire purification scheme be completed as rapidly as possible. However, the number of purification steps makes a demanding schedule that may not always be practical. If the purification steps are carried out as quickly as possible, the time from the end of overnight expression through to the 2-4 hours of incubation at 30°C with TEV protease is approximately 9-11 hours. One potential place to pause is following the TEV cleavage step. If absolutely necessary, Hsp104 may be snap frozen after this step as described above (Step 5.2). Size-exclusion chromatography (Step 3.9) is then performed immediately after thawing and the purified Hsp104 must then be used immediately for biochemical assays. Additionally the induction step can also be streamlined to 2 hours at 37°C although this reduces overall yield.
Potential complications: This purification scheme is straight-forward. However, a few critical steps and alternative procedures are discussed below.
Alternative cell lysis: If a French Press is not available, then lysozyme treatment followed by sonication is an effective cell lysis method. Lysis buffer-resuspended cells are incubated with 20mg of hen egg lysozyme (Sigma) per 1L-cell pellet for 30 min on ice to a final concentration of 2mg/ml lysozyme. Cells are then sonicated for two 30 second bursts at level 9 using a MisonixSonicator 3000 with a microtip. The cell suspension is incubated on ice for 1min in between sonication bursts. Lysis has occurred when the suspension changes viscosity and color. Cell debris is then removed as described above (Step 2.4). French press lysis is preferred to sonication because this minimizes Hsp104 degradation and loss of Hsp104 activity.
TEV Protease Cleavage: Curiously, the pPROEX-Htb-Hsp104 His6 tag is particularly resistant to cleavage by TEV protease and requires approximately twice the amount of TEV protease recommended by the manufacturer. Removal of this tag is essential as the pPROEX-Htb His6-tag interferes with Hsp104 disaggregase activity if it is not completely removed. If fully functional His6-tagged Hsp104 is required (e.g. for depletion experiments26) we recommend using the His6-tagged Hsp104 construct described by Schirmer and Lindquist47, where the His6-tag does not affect disaggregase activity26. This protein can be purified using the protocol described above except the TEV cleavage step is omitted.
The protocol we have outlined above is more facile than previous approaches26,47,48. The rapidity and decreased number of steps allows for less perturbation of the protein and therefore yields highly purified and active Hsp104 suitable for structural and mechanistic studies.
No conflicts of interest declared.
This work was supported by a grant from the NIH (5T32GM008275-22) and an American Heart Association predoctoral fellowship (to E.A.S.); a Chemistry-Biology Interface Fellowship from the NIH (2T32GM071339-06A1) (to M.E.D.); and grants from the NIH (1DP2OD002177-01 and NS067354-0110), The Ellison Medical Foundation, and The Bill and Melinda Gates Foundation (to J.S.).
|BL21-CodonPlus-RIL Competent Cells||Stratagene, Agilent Technologies||230255|
|2XYT broth||USB Corp., Affymetrix||75864|
|Complete, mini, EDTA-free protease inhibitor tablets||Roche Group||1836170|
|Ni-Sepharose 6 Fast Flow||GE Healthcare||17-5318-02|
|Amicon Ultra-15 centrifugal filter units (MWCO 30,000)||EMD Millipore||UFC903008|
|Resource Q - 6ml column||GE Healthcare||17-1179-01|
|proTEV Protease||Promega Corp.||V6052|
|Superose 6 10/300 GL||GE Healthcare||17-5172-01|
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