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Journal / Biology / Hsp104の精製、タンパク質Disaggregase
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Hsp104の精製、タンパク質Disaggregase
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Hsp104の精製、タンパク質Disaggregase

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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ここで、我々は、高活性Hsp104、六量体AAA +タンパク質酵母、タンパク質の分解に結合ATPの加水分解からの精製のためのプロトコルについて説明します。このスキームでは、からアフィニティー精製のためのしたHis6 -タグ付き構造を活用して

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分子生物学、問題55、神経科学、Hsp104、AAA +、disaggregase、熱ショック、アミロイド、プリオン
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