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Chromatographische Reinigung von hoch aktiven Hefe Ribosomen
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Chromatographische Reinigung von hoch aktiven Hefe Ribosomen

Article DOI: 10.3791/3214-v • 09:14 min • October 24th, 2011
October 24th, 2011 •
1,2, 1, 1
1Department of Cell Biology and Molecular Genetics, University of Maryland, 2Department of Biotechnology and Microbiology, Vilnius University

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Die Kontamination von Zubereitungen der eukaryotischen Ribosomen mit herkömmlichen Methoden durch Co-Reinigung Nukleasen und Proteasen gereinigt negativ auf die nachgelagerten biochemische und strukturelle Analysen. Eine schnelle und einfache chromatographische Reinigungsverfahren wird verwendet, um dieses Problem unter Verwendung von Hefe Ribosomen als Modellsystem zu lösen.

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Cell Biology Ausgabe 56 Ribosomen Reinigung DNA Hefe Chromatographie,
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