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Medicine
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采用TaqMan从儿童凳诺如病毒的检测和Genogrouping一步法RT-PCR技术
 
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采用TaqMan从儿童凳诺如病毒的检测和Genogrouping一步法RT-PCR技术

Article DOI: 10.3791/3232-v 15:17 min July 22nd, 2012
July 22nd, 2012

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Summary

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Click here for the English version.

一个一步法RT-PCR方法检测检测和孩子的大便,,利用引物和的TaqMan探针的开放阅读框(ORF1基因)的病毒ORF2交界地区,对诺沃克类病毒基因组中最保守的地区是特定genogroup诺沃克病毒分离鉴定描述。详述一个非商业化,具有成本效益的RNA提取方法。

Tags

病毒学,65岁,医学,遗传学,诺罗病毒,胃肠炎,RNA提取,腹泻,粪便样品,PCR扩增RT-PCR,TaqMan探针,硅胶
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