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A realização de experimentos de Microarray personalizado MicroRNA
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A realização de experimentos de Microarray personalizado MicroRNA

Article DOI: 10.3791/3250-v • 07:05 min • October 28th, 2011
October 28th, 2011 •
1, 1,2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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Um procedimento simples de realizar experimentos de microarray personalizado microRNA é descrito. As etapas incluem isolar RNA, rotulagem RNA e DNA de referência, hibridação as amostras para microarrays, a digitalização da microarrays, quantificar e analisar os sinais de hibridização.

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Biologia Molecular microRNA microarray personalizado sondas de oligonucleotídeo RNA rotulagem
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