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Performing Benutzerdefinierte MicroRNA Microarray-Experimenten
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Performing Benutzerdefinierte MicroRNA Microarray-Experimenten

Article DOI: 10.3791/3250-v • 07:05 min • October 28th, 2011
October 28th, 2011 •
1, 1,2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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Ein einfaches Verfahren zur Durchführung benutzerdefinierte microRNA Microarray-Experimenten beschrieben. Die Schritte umfassen Isolierung von RNA, die Kennzeichnung RNA und DNA-, Hybridisierung der Proben auf Microarrays, Scannen der Microarrays, Quantifizierung und Analyse Hybridisierungssignale.

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Molecular Biology Ausgabe 56 microRNA kundenspezifische Microarrays Oligonukleotid-Sonden RNA-Markierung
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