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Udførelse Brugerdefineret MicroRNA Microarray Eksperimenter
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Udførelse Brugerdefineret MicroRNA Microarray Eksperimenter

Article DOI: 10.3791/3250-v • 07:05 min • October 28th, 2011
October 28th, 2011 •
1, 1,2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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En simpel procedure til at udføre brugerdefinerede microRNA microarray eksperimenter er beskrevet. Trinene omfatter isolere RNA, mærkning RNA og reference DNA, hybridizing prøverne til microarrays, scanning af microarrays, kvantificere og analysere hybridisering signaler.

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Molekylær Biologi Genetik microRNA brugerdefineret microarray oligonukleotid sonder RNA mærkning
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