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Visualisering af DNA-replikation i hvirveldyr modelsystem DT40 ved hjælp af DNA-Fiber Teknik
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Visualisering af DNA-replikation i hvirveldyr modelsystem DT40 ved hjælp af DNA-Fiber Teknik

Article DOI: 10.3791/3255-v • 07:18 min • October 27th, 2011
October 27th, 2011 •
1, 1,2
1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, University of Oxford, 2Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw

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DT40, en model hvirveldyr genetiske system, giver et kraftfuldt værktøj til at analysere proteinernes funktion. Her beskriver vi en simpel metode, der giver mulighed for kvalitativ analyse af de parametre, der påvirker DNA-syntesen i S-fase i DT40 celler på enkelt molekyle niveau.

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Molekylær Biologi genetik DNA fiber analyse replikering hastighed gaffel i stå oprindelse fyring opsigelse
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