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JoVE Journal Neuroscience
Bilaminar Co-kultur Primär Rat kortikala neuron och Glia
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Bilaminar Co-kultur Primär Rat kortikala neuron och Glia

Article DOI: 10.3791/3257-v • 12:33 min • November 12th, 2011
November 12th, 2011 •
*1, *1, 1
1Department of Pharmacology and Physiology, Drexel University College of Medicine
* These authors contributed equally

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Här ger vi ett protokoll för kortikala odling råtta neuron i närvaro av en stödjeceller feeder lager. Den odlade nervceller skapa polaritet och skapa synapser och kan separeras från Glia för användning i olika applikationer, såsom elektrofysiologi, kalcium bildhantering, cell analyser överlevnad, immuncytokemiundersökning och RNA / DNA / protein isolering.

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Neurovetenskap neuron råtta hjärna co-kultur cortex Glia gliaceller
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