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JoVE Journal
Biology

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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
 

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Article DOI: 10.3791/3265-v 12:01 min February 20th, 2012
February 20th, 2012

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Summary

We provide a cost-effective and rapid molecular genotyping protocol that employs variety-specific PCR primers that target DNA sequence differences within the chloroplast trnL-F spacer region to differentiate between varieties of Imperata cylindrica (cogongrass) that cannot be distinguished by morphology alone. These varieties include the federally listed noxious weed, cogongrass and closely-related, wide-spread ornamental variety, I. cylindrica var. koenigii (Japanese blood grass).

Tags

PCR-based Genotyping Method Wild-type Ornamental Varieties Imperata Cylindrica Cogongrass Invasive Plants Native Plant Species Ornamental Variety I. Cylindrica Var. Koenigii Rubra Red Baron Japanese Blood Grass JBG Sterile And Noninvasive Viable Seed Production Green Invasive Form Morphology Identification Plant Taxonomists Sequence Comparisons Coding Regions Variable Regions Nuclear DNA Chloroplast DNA
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