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Journal / Immunology and Infection / Un protocollo Chelex semplice per…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Un protocollo Chelex semplice per l'estrazione del DNA da
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Un protocollo Chelex semplice per l'estrazione del DNA da

Article DOI: 10.3791/3281-v • 07:16 min • January 9th, 2013
January 9th, 2013 •
1, 1, 1, 1, 2, 1, 1
1Malaria Institute at Macha, 2Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health

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Un modo rapido e conveniente per estrarre parassita della malaria qualità e il DNA da campioni di zanzara vettore è descritto. Capitalizzando proprietà chelanti di resina Chelex, il semplice metodo consente genotipizzazione dei parassiti della malaria in fasi zanzara ghiandole mid-intestinali e salivare, nonché identificazione molecolare del

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Infezione Numero 71 Immunologia Malattie Infettive genetica biologia molecolare microbiologia parassitologia entomologia malaria, Vettore, Ditteri zanzare Chelex DNA estrazione PCR dissezione insetto vettore agente patogeno
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