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Enkelt Læs og Forbundne End mRNA-Seq Illumina Biblioteker fra 10 nanogram Total RNA
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Enkelt Læs og Forbundne End mRNA-Seq Illumina Biblioteker fra 10 nanogram Total RNA

Article DOI: 10.3791/3340-v • 14:50 min • October 27th, 2011
October 27th, 2011 •
1, 1, 1, 1, 1,2,3, 1, 1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

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Her beskriver vi en metode til klargøring af både enkelt læser og parret ende Illumina mRNA-Seq sekvensering biblioteker til genekspressionsanalyse baseret på T7 lineær RNA forstærkning. Denne protokol kræver kun 10 nanogram at starte total RNA og genererer meget konsekvent biblioteker repræsenterer hele udskrifter.

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Molekylær Biologi Genetik mRNA-Seq Illumina-Seq genekspression profilering high throughput sekventering
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