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Solo lectura y pareadas final Sec ARNm bibliotecas de Illumina de 10 nanogramos ARN total
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Solo lectura y pareadas final Sec ARNm bibliotecas de Illumina de 10 nanogramos ARN total

Article DOI: 10.3791/3340-v • 14:50 min • October 27th, 2011
October 27th, 2011 •
1, 1, 1, 1, 1,2,3, 1, 1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

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Aquí se describe un método para la preparación de los dos pares de solo lectura y final Illumina Sec-mRNA bibliotecas genómicas para el análisis de expresión genética basado en la amplificación del ARN T7 lineal. Este protocolo requiere solamente 10 nanogramos de ARN total a partir bibliotecas y genera muy consistente que representa transcripciones conjunto.

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Biología molecular número 56 la genética el ARNm-Seq Illumina-Seq perfiles de expresión génica secuenciación de alto rendimiento
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