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Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplex Påvisning av bakterier i Complex Clinical and Environmental Prøver å bruke oligonukleotid-coupled Fluorescent Mikrokuler
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Multiplex Påvisning av bakterier i Complex Clinical and Environmental Prøver å bruke oligonukleotid-coupled Fluorescent Mikrokuler

Article DOI: 10.3791/3344-v • 11:09 min • October 23rd, 2011
October 23rd, 2011 •
1, 1, 2, 2, 3
1Saskatoon Research Centre, Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology, University of Saskatchewan, 3Plant Biotechnology Institute, National Research Council of Canada

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Vi beskriver en multiplex metode for påvisning av mikroorganismer i en prøve med oligonukleotid-kombinert fluoriserende perler. Amplicon fra alle organismer innen en prøve er hybridisert til et panel av probe-koblet perler. En Luminex eller Bio-Plex instrumentet brukes til å spørre hver perle for perle type og hybridisering signal.

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Immunologi medisin chaperonin-60 hsp60 Luminex multiplex diagnostikk bakteriell vaginose PCR
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