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Journal / Bioengineering / Parallell-plate Flow Chamber og kontinuerlig flyt …
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Bioengineering

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JoVE Journal Bioengineering
Parallell-plate Flow Chamber og kontinuerlig flyt Circuit å evaluere endoteliale progenitorceller henhold Laminar Flow skjærspenning
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Parallell-plate Flow Chamber og kontinuerlig flyt Circuit å evaluere endoteliale progenitorceller henhold Laminar Flow skjærspenning

Article DOI: 10.3791/3349-v • 12:43 min • January 17th, 2012
January 17th, 2012 •
1, 2, 2, 3, 1, 1, 2, 2, 1, 4, 2, 1
1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center

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Vi beskriver en metode å utsette tilhenger celler til laminær skjærspenning i et sterilt kontinuerlig flyt krets. Cellenes vedheft, kan morfologi bli undersøkt gjennom de gjennomsiktige kammer, prøver hentet fra kretsen for metabolitten analyse og celler høstet etter shear eksponering for fremtidige eksperimenter eller kultur.

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Bioteknologi Fluid skjærspenning skjærspenning skjærkraft endotelet endoteliale progenitorceller Flow Chamber Laminar Flow Flow Circuit Continuous Flow celle adhesjon
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