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JoVE Journal Bioengineering
Rotierende Cell Culture Systems für Human Cell Culture
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Rotierende Cell Culture Systems für Human Cell Culture: Human Trophoblastzellen als Modell

Article DOI: 10.3791/3367-v • 06:54 min • January 18th, 2012
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Traditional, zweidimensionalen Zellkultur-Techniken oft in veränderten Eigenschaften in Bezug auf die Differenzierung Marker, Zytokine und Wachstumsfaktoren führen. Dreidimensionale Zellkulturen in den rotierenden Zellkultursystem (RCCS) wiederherstellt Expression vieler dieser Faktoren, wie hier mit einem extravillösen Trophoblasten-Zelllinie gezeigt.

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Bioengineering extravillösen Trophoblasten Zytotrophoblasten Invasion Matrixmetalloproteinase 3-D Zellkultur RCCS ECM Mikroträger
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