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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Article DOI: 10.3791/3370-v • 10:32 min • January 16th, 2012
January 16th, 2012 •
1, 1, 1
1School of Biotechnology, Department of Proteomics, Royal Institute of Technology

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A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.

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Orthogonal Protein Purification Small Bispecific Affinity Tag Recombinant Proteins Purification Protocols High-throughput Methods Purification Tags Hexa-histidine Tag Affinity Chromatography Bispecific Purification Tag Albumin-binding Domain Streptococcal Protein G Human Serum Albumin (HSA) Phage Display Technology Dimeric Z-domain Staphylococcal Protein A
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