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JoVE Journal
Biology

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使用油脂全线Bicelle方法的膜蛋白的高通量结晶
 
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使用油脂全线Bicelle方法的膜蛋白的高通量结晶

Article DOI: 10.3791/3383-v 07:26 min January 9th, 2012
January 9th, 2012

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

Bicelles脂质/两亲分子的混合物保持在脂质双层膜蛋白(MPS),但有独特的相行为,有利于高通量筛选的结晶机器人。这项技术已成功地生产从原核和真核来源的高分辨率结构。本视频介绍了生成油脂全线Bicelle混合物,纳入全线Bicelle混合物的国会议员,成立从中长期的结晶试验(手动以及作为机器人)和收割晶体的协议。

Tags

分子生物学,59期,膜蛋白结晶,全线Bicelle,油脂结晶
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