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High-throughput krystallisering af membranproteiner Brug af Lipidic Bicelle Metode
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High-throughput krystallisering af membranproteiner Brug af Lipidic Bicelle Metode

Article DOI: 10.3791/3383-v • 07:26 min • January 9th, 2012
January 9th, 2012 •
1, 2
1UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles, 2Department of Physiology, David Geffen School of Medicine, UCLA

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Bicelles er lipid / amphiphile blandinger, der opretholder membranproteiner (MP) inden for en tolagede men har unikke fase adfærd, der fremmer high-throughput screening ved krystallisation robotter. Denne teknik har med succes produceret en række høj opløsning strukturer fra både prokaryote og eukaryote kilder. Denne video beskriver protokoller for at generere lipidic bicelle blandingen, som omfatter parlamentsmedlemmer i bicelle blandingen, etablering krystalliseringer forsøg (såvel manuelt som robotersvejsede) og høste krystaller fra mediet.

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Molekylær Biologi membranproteiner krystallisering bicelle lipidic krystallisering
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