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Selektieren und Isolieren Colonies of Human induzierten pluripotenten Stammzellen aus adulten Fibroblasten Umprogrammierte
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Selektieren und Isolieren Colonies of Human induzierten pluripotenten Stammzellen aus adulten Fibroblasten Umprogrammierte

Article DOI: 10.3791/3416-v • 13:24 min • February 20th, 2012
February 20th, 2012 •
1,2, 1, 3, 3, 1, 1
1Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, 2Department of Cell Biology, Poznan University of Medical Sciences, 3Department of Molecular Biology, The Scripps Research Institute

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Wir präsentieren ein Protokoll für die effiziente Reprogrammierung von menschlichen Körperzellen in die menschlichen induzierten pluripotenten Stammzellen (hiPSC) mit retroviralen Vektoren kodieren Oct3 / 4, Sox2, Klf4 und c-Myc (OSKM) und Identifizierung von richtig umprogrammiert hiPSC durch Live-Färbung mit Tra- 1-81 Antikörper ist.

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Developmental Biology Ausgabe 60 Stammzellen induzierte pluripotente Stammzellen IPSC Reprogrammierung somatischer Zellen Pluripotenz retrovirale Transduktion
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