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Välja och isolera Kolonier av mänskliga inducerade pluripotenta stamceller omprogrammeras från vuxna fibroblaster
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Välja och isolera Kolonier av mänskliga inducerade pluripotenta stamceller omprogrammeras från vuxna fibroblaster

Article DOI: 10.3791/3416-v • 13:24 min • February 20th, 2012
February 20th, 2012 •
1,2, 1, 3, 3, 1, 1
1Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, 2Department of Cell Biology, Poznan University of Medical Sciences, 3Department of Molecular Biology, The Scripps Research Institute

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Vi presenterar ett protokoll för effektiv omprogrammering av mänskliga somatiska celler till humana inducerade pluripotenta stamceller (hiPSC) med retrovirusvektorer som kodar Oct3 / 4, Sox2, Klf4 och c-myc (OSKM) och identifiering av korrekt omprogrammeras hiPSC med levande färgning med Tra- 1-81 antikropp.

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Developmental Biology stamceller inducerade pluripotenta stamceller IPSC somatisk cellterapi omprogrammering pluripotens retroviral transduktion
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