Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).
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Doan, T. T., Freeman, M. H., Schmidt, A. R., Nguyen, N. D., Leopold, M. C. Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry. J. Vis. Exp. (56), e3441, doi:10.3791/3441 (2011).
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Colloidal gold nanoparticles protected with alkanethiolate ligands called monolayer protected gold clusters (MPCs) are synthesized and subsequently incorporated into film assemblies that serve as adsorption platforms for protein monolayer electrochemistry (PME). PME is utilized as the model system for studying electrochemical properties of redox proteins by confining them to an adsorption platform at a modified electrode, which also serves as a redox partner for electron transfer (ET) reactions. Studies have shown that gold nanoparticle film assemblies of this nature provide for a more homogeneous protein adsorption environment and promote ET without distance dependence compared to the more traditional systems modified with alkanethiol self-assembled monolayers (SAM).1-3 In this paper, MPCs functionalized with hexanethiolate ligands are synthesized using a modified Brust reaction4 and characterized with ultraviolet visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and proton (1H) nuclear magnetic resonance (NMR). MPC films are assembled on SAM modified gold electrode interfaces by using a "dip cycle" method of alternating MPC layers and dithiol linking molecules. Film growth at gold electrode is tracked electrochemically by measuring changes to the double layer charging current of the system. Analogous films assembled on silane modified glass slides allow for optical monitoring of film growth and cross-sectional TEM analysis provides an estimated film thickness. During film assembly, manipulation of the MPC ligand protection as well as the interparticle linkage mechanism allow for networked films, that are readily adaptable, to interface with redox protein having different adsorption mechanism. For example, Pseudomonas aeruginosa azurin (AZ) can be adsorbed hydrophobically to dithiol-linked films of hexanethiolate MPCs and cytochrome c (cyt c) can be immobilized electrostatically at a carboxylic acid modified MPC interfacial layer. In this report, we focus on the film protocol for the AZ system exclusively. Investigations involving the adsorption of proteins on MPC modified synthetic platforms could further the understanding of interactions between biomolecules and man-made materials, and consequently aid the development of biosensor schemes, ET modeling systems, and synthetic biocompatible materials.5-8
1. Hexanethiolate Monolayer Protected Gold Clusters Synthesis
Hexanethiolate functionalized monolayer protected gold clusters (MPCs) are synthesized following a 2:1 1-hexanethiol (C6) to gold mole ratio to produce an average structure of Au225(C6)75. 4-9 Speciﬁc modiﬁcations to the Brust reaction, like ligand type, speciﬁc thiol-to-gold ratios, temperature, and reaction delivery rate, or post-synthesis treatments, 9-11 can yield a diverse range of MPCs with varying core sizes and functional protective groups, respectively.4 The MPC approximate (average) compositions of MPCs functionalized with various alkanethiol groups can be determined by proton (1H) nuclear magnetic resonance (NMR) analysis of iodine-decomposed samples.
- Dissolve 1.1 g tetraoctylammonium bromide (TOABr) in 30 mL of toluene with appropriate fume hood ventilation.
- Dissolve 0.38 g sodium borohydride (NaBH4) in ˜20 mL of 18 MΩ ultrapuriﬁed water (UP H2O) and allow to chill over ice for at least 30 min.
- Dissolve 0.31 g hydrogen tetrachloroaurate (HAuCl4) in ˜20 mL of UP H2O and quantitatively transfer the solution mixture to the TOABr-toluene solution using an additional ˜5 mL of UP H2O in order to phase-transfer the aqueous gold solution to the nonaqueous solution. Stir rigorously while lightly capped for 30 min so the burnt orange aqueous and clear nonaqueous phases are mixing well.
- Transfer both the clear aqueous and burnt orange nonaqueous phases to a separatory funnel. Discard the aqueous (bottom) layer and decant the nonaqueous (top) layer into a clean flask.
- Add C6 in a ratio of 2:1 with HAuCl4 to the nonaqueous solution. Stir for 30 min to form a Au(I) polymer, as detected by a color change from reddish orange to a pale yellow, nearly colorless solution.
- Transfer the reaction mixture to an insulated ice bath and chill to 0°C for at least 30 min with stirring.
- Quantitatively and quickly add the chilled NaBH4 solution to the reaction mixture in order to reduce Au(I) to a metallic gold in the presence of thiols, instantaneously forming a thick black solution of MPCs upon addition. Stir reaction overnight at 0°C.
- Transfer the reaction mixture to a separatory funnel, discard the aqueous (bottom) layer into a waste beaker, and rotary evaporate the nonaqueous (top) toluene layer to near complete dryness leaving a heavy black sludge in the flask.
- Precipitate the MPCs by adding acetonitrile and allowing to sit overnight.
- Collect MPCs by vacuum filtration using a glass frit of medium porosity with rubber fittings and side-armed flask with aspirator and rinse with a copious amount of acetonitrile.
- Allow MPCs to air dry, weigh product, characterize by transmission electron microscopy (TEM) and NMR analysis, and store capped for future use. Obtain TEM images by drop-casting MPCs dissolved in toluene onto formvar/carbon support film on copper grid (400 mesh) and operating the TEM instrument at 80-100 kV. The average core size can be estimated using image analysis software such as Image J (freeware).
2. Film Assembly: Dithiol-linked MPC Film Assembly for Protein Monolayer Electrochemistry
The gold substrate is first electrochemically cleaned and modified with a C6 SAM before immersing in alternating solutions of dithiol linking molecules and C6 modified MPCs to make up a "dip cycle," which is repeated several times to ultimately form a dithiol-linked MPC film assembly. As described in prior studies,2 the original plasmid for the Pseudomonas aeruginosa azurin (AZ) protein was graciously given by Dr. Corey Wilson of Rice University and AZ was provided as a purified and lyophilized powder by the University of Richmond professor, Dr. Jonathan Dattelbaum, that was subsequently rehydrated with 4.4 mM potassium phosphate buffer (KPB, pH = 7.0, μ = 10 mM) to create a 5-10 μM solution as verified by ultraviolet visible (UV-Vis) analysis.
- Assemble the electrochemical (echem) sandwich cell in the following order from bottom to top: first Lucite retainer plate, gold substrate as a working electrode, brass electrical contact to gold working electrode, first rubber gasket, Viton o-ring that defines the electrode area (0.32 cm2), glass cell body, second rubber gasket, and second Lucite retainer plate. The entire cell is held together by threaded rods and carefully tightened wing nuts. The cell is fitted with a commercially purchased reference electrode that houses a glass barrel with 1 M saturated KCl, Ag/AgCl reference wire, and a Pt auxiliary electrode wire.
- Electrochemically clean the gold substrate by performing cyclic voltammetry (CV) in the potential windows from 0.2 to 0.9 V, 0.2 to 1.2 V, and 0.2 to 1.35 V (versus Ag/AgCl, KCl) at 100 mV/s in a solution of 0.1 M H2SO4 and 0.01 M KCl.
- Measure the charging current of the cleaned bare gold substrate by performing CV at "standard conditions," including a potential window from 0.1 to 0.4 V (versus Ag/AgCl, KCl) scanned at 100 mV/s in KPB.1 Discard KPB and rinse successively with UP H2O, ethanol (EtOH), UP H2O, and EtOH.
- Expose the cleaned gold substrate to ˜300 μl of 5 mM C6 solution in EtOH and allow to sit overnight to form an ordered C6 SAM. Discard C6 solution from the cell and rinse successively with EtOH, UP H2O, EtOH, and UP H2O.
- Measure the charging current of the SAM at standard conditions. Discard KPB and rinse successively with UP H2O, EtOH, UP H2O, and EtOH. The charging current should be markedly decreased from that of the bare gold measurement (step 2.3).1
- Expose the SAM modified gold substrate to ˜300 μl of 5 mM 1,9-nonanedithiol (NDT) solution in EtOH and allow to sit for 1 hr to interdisperse NDT linking molecules within the C6 SAM. Discard NDT solution and rinse successively and thoroughly with EtOH, UP H2O, EtOH, UP H2O, and methylene chloride (CH2Cl2).
- Expose the gold substrate to a MPC solution of CH2Cl2 (˜1 mg/mL) with agitation by slowly bubbling with N2 gas for 1 hr. If necessary, replace evaporated MPC solution with more CH2Cl2. This is the anchoring MPC layer of the film assembly. Discard MPC solution and again rinse successively with CH2Cl2, UP H2O, and KPB.
- Measure the charging current of the MPC layer at standard conditions. Discard KPB and rinse successively with UP H2O and CH2Cl2.
- Expose the gold substrate to ˜300 μl of 5 mM NDT solution of CH2Cl2 with agitation by slowly bubbling with N2 gas for 20 min.
- Discard NDT and rinse thoroughly with CH2Cl2. Repeat steps 2.7 and 2.8 to deposit the second MPC layer of the film assembly.
- To deposit additional MPC layers, steps 2.9 and 2.10 are repeated. With each additional MPC layer a corresponding increase in the charging current is observed.
- After the networked MPC film is complete, rinse the film modified substrate with KPB. AZ protein is adsorbed on the MPC film assembly by injecting ˜150 μL of ˜5-10 μM AZ solution of KPB into the echem sandwich cell and allowing to sit capped and refrigerated for at least 1 hr.
- Remove the echem cell from the refrigerator and allow it to return to near room temperature. Thoroughly rinse with KPB, refill echem cell with KPB, and bubble KPB with N2 gas for 10 min.
- Protein monolayer electrochemical studies are performed as CV in the potential window from -0.25 V to 0.25 V (versus Ag/AgCl, KCl) scanned at 100 mV/sec in KPB.
3. Film Assembly: Dithiol-linked MPC Film Assembly for Optical Tracking
Prior to growing MPC films for optical evaluation, glass slide sections are pre-cleaned with Piranha solution (CAUTION! 2:1 concentrated H2SO4 and H2O2) and treated with (3-mercaptopropyl)-trimethoxysilane (3-MPTMS).1-2 MPC films are then assembled on these modified glass slides using the "dip cycle" technique as previously described above.
- Rinse a 3-MPTMS modified glass slide with CH2Cl2 and place it in a MPC solution of CH2Cl2 (˜1 mg/mL) for 1 hr while agitating on a shaker at low speed. This completes the first MPC layer of the film assembly by anchoring MPCs to the mercaptans endgroups of the silane. Rinse the slide thoroughly with CH2Cl2, and dry with N2 gas. Take a UV-Vis spectrum (from 400 to 1000 nm) of the slide, and rinse again with CH2Cl2.
- Place the slide in a 5 mM NDT solution of CH2Cl2 for 1 hr while agitating on a shaker at low speed. Rinse the slide with CH2Cl2.
- Place the slide in a MPC solution for 1 hr while agitating on a shaker at low speed. This completes the second MPC layer of the film assembly. Rinse the slide thoroughly with CH2Cl2, dry with N2 gas, and take a UV-Vis spectrum (from 400 to 1000 nm) of the slide. The absorbance across the spectrum should be increasing as additional MPC layers are adsorbed to the film assembly.
- To deposit additional MPC layers, steps 3.2-3.3 are repeated.
4. Characterization of Monolayer Protected Gold Cluster Film Assemblies by Cross Sectional Transmission Electron Microscopy
TEM cross sections are prepared by re-embedding en face embedded films.2, 12 This is done by first attaching a MPC film assembled on a 3-MPTMS modified glass slide onto a clean, standard microscope slides using Embed 812 epoxy resin to allow for improved handling during the procedure below. Use caution with the applied heat as higher temperatures will decompose the MPCs within the film.
- Mix Embed 812 epoxy resin and allow to thicken for at least 12 hr.
- Fill a "00" BEEM capsule with epoxy resin and invert on top of the MPC film sample (prepared in section 3). Place pressure on the capsule so that a bubble rises to the top of capsule, creating a seal between the epoxy resin and MPC film sample. Allow to polymerize for at least 18 hr at 60°C and then cool the mounted slides to room temperature.
- Heat mounted slides for 20 sec on a cast aluminum hot plate at 200°C in order to facilitate the removal of the block with attached MPC en face film.
- Cut the sample containing the film off of the BEEM capsule block using a jeweler's saw.
- Re-embed the removed portion in a silicon flat mold with the MPC film side up facing the interior of the silicon well. Fill the silicon well with epoxy resin at room temperature and allow to polymerize for at least 18 hr at 60°C. Cool the sample to room temperature.
- Obtain thin sample sections of 60-80 nm on a Leica UCT ultramicrotome by using a diamond knife to cut sections perpendicular to the knife's edge.
- Place sliced sections on formvar/carbon support film on copper grid (400 mesh) and take TEM images of prepared cross sections of MPC film assemblies.
5. Representative Results:
Figure 1 Double layer charging current monitoring of MPC film growth for a total of 5 dipping cycles (alternating exposure to MPC and NDT solutions). Charging current increased systematically with each dipping cycle, adding "layers" of MPC to the film (Fig. 2). The cyclic voltammograms were collected using a potential window from 0.1 to 0.4 V (versus Ag/AgCl, KCl) scanned at 100 mV/s in 4.4 mM potassium phosphate buffer (pH = 7.0, μ = 10 mM). Reprinted with permission from M.L. Vargo, C.P. Gulka, J.K. Gerig, C.M. Manieri, J.D. Dattelbaum, C.B. Marks, N.T. Lawrence, M.L. Trawick, and M.C. Leopold, Langmuir 26(1), 560-569. Copyright 2010 American Chemical Society.
Figure 2 (a) Schematic representation of AZ protein adsorbed to a dithiol-linked MPC film assembly. (b) Typical cyclic voltammogram for AZ adsorbed to MPC film assembly collected using a potential window from -0.25 to +0.25 V (versus Ag/AgCl, KCl) scanned at 100 mV/s in 4.4 mM potassium phosphate buffer (pH = 7.0, μ = 10 mM).
Figure 3 Representative UV-Vis spectral monitoring of a dithiol-linked MPC film growth on a 3-MPTMS modified glass slide. A dip cycle consists of an exposure of the glass slide to NDT linker solution followed an exposure to MPC solution. Each subsequent dip results in growth in film thickness and a concurrent absorbance increase. As the number of dip cycles increases, the surface plasmon band is gradually defined at ˜520 nm. Reprinted with permission from M.L. Vargo, C.P. Gulka, J.K. Gerig, C.M. Manieri, J.D. Dattelbaum, C.B. Marks, N.T. Lawrence, M.L. Trawick, and M.C. Leopold, Langmuir 26(1), 560-569. Copyright 2010 American Chemical Society.
Figure 4 Transmission electron microscopy (TEM) cross sectional image analysis of a dithiol-linked MPC film assembly. Inset: Typical TEM image of hexanethiolate functionalized MPCs used in the film assembly. TEM analysis determined an average gold core diameter of the MPCs to be ˜2 nm using Image J analysis. Reprinted with permission from M.L. Vargo, C.P. Gulka, J.K. Gerig, C.M. Manieri, J.D. Dattelbaum, C.B. Marks, N.T. Lawrence, M.L. Trawick, and M.C. Leopold, Langmuir 26(1), 560-569. Copyright 2010 American Chemical Society.
Protein monolayer electrochemistry is an effective technique used to study interactions between redox proteins and synthetic adsorptive platforms. The effectiveness of this strategy, however, is dependent on the ability to engineer an adsorption interface with a high degree of molecular level control. The MPC-based platforms created by this protocol represent specifically engineered platforms that are able to provide a more homogenous protein adsorption environment3 and facilitate ET over a greater distance2 compared to traditional PME systems employing alkanethiolate SAMs. A strength of the MPC film assembly as an electrochemical interface is its versatility and adaptability to other redox proteins of different size and surface chemistry/function, various different nanomaterials, and alternate electrode configurations as well. For example, the described procedure is readily adapted to ET studies of cytochrome c (cyt c) by using simple place-exchange reactions on the outermost layer of MPCs incorporated into the assembly.11 As cyt c is cationic and is able to bind to substrates electrostatically, carboxylic acid-terminated alkanethiols thiols can be place-exchanged into the peripheral ligands of MPCs comprising the modified electrode interface to facilitate an electrostatic driven immobilization of the protein, with the subsequent electrochemical analysis being identical to that described here.1 To adjust the size of the MPCs to accommodate different sizes of proteins, adjustments to the Brust synthesis, such as changing thiol-to-gold ratios, reaction temperature/delivery rate, yield a wide range of MPC diameters that will match the approximate diameter of a targeted protein.9-10
The general procedure, primarily repetitive cycles of exposure to particles and linking molecules (layer-by-layer) has been successfully used to create thin films incorporating a variety of different nanomaterials. For example, aqueous nanoparticles (NPs) with different protective coatings and unique optical properties have been networked into films that are linked exclusively with electrostatic interactions between NPs and polyelectrolyte bridges.13 The same strategy has also been applied to the construction of highly optically sensitive film assemblies featuring nanoshell or hollow NPs.
While the procedure described here utilizes custom designed electrochemical cells and gold substrates, it is readily adaptable to more generic electrodes, electrochemical configurations, and electroanalytical techniques. In addition to all the films described being able to be constructed on evaporated gold electrodes and glass slides, the films have also been easily assembled on common gold disk electrodes that are readily available from CH Instruments or Bioanalytical Systems (BAS). Though cyclic voltammetry continues to be the primary electrochemical technique in PME, we have recently successfully analyzed protein monolayer ET with a variety of other electrochemical techniques, including step, pulse, and impedance based techniques.14
Research and development of nanomaterial-based interfaces for protein adsorption are ongoing but the MPC film assemblies described in this report represent an effective and improved strategy for PME studies. The procedure is relatively straightforward and can be performed by students and scientists of all levels, creating highly versatile films that can be easily tailored to specific protein targets if necessary.
No conflicts of interest declared.
We gratefully acknowledge the National Science Foundation (CHE-0847145), and the Henry Dreyfus Teacher-Scholar Awards Program for generously supporting this research. We would like to specifically recognize Christine Davis, Director of Microscopy and Imaging in the Department of Biology at the University of Richmond for her assistance with cross-sectional imaging. Special thanks is given to Drs. T. Leopold, R. Kanters, D. Kellogg, R. Miller, and W. Case, as well as, Russ Collins, Phil Joseph, Carolyn Marks, Mandy Mallory and John Wimbush – all of whom make undergraduate research possible at the University of Richmond. A very personal thank you is given to all current, past, and future undergraduate researchers in the Leopold Research Lab.
|Transmission Electron Microscope||JEOL||1010|
|Nuclear Magnetic Resonance Spectrometer||Bruker Corporation||300 MHz|
|Formvar/carbon support film on copper grid (400 mesh)||Electron Microscopy Sciences||FCF400-Cu|
|Gold substrate||Evaporated Metal Films Corp.||Custom|
|Ag/AgCl Reference electrode||Microelectrodes, Inc.||MI-401F|
|Potentiostats||CH Instruments, Inc.||CHI650A, CHI610B|
|Ultraviolet Visible Spectrophotometer||Agilent Technologies||8453|
|Embed 812 epoxy resin||Electron Microscopy Sciences||14120|
|"00" BEEM capsule||Electron Microscopy Sciences||70000-B|
|Silicon flat mold||Electron Microscopy Sciences||70900|
|Diamond knife||Diatome||21-ULE, S12801|
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