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Het meten van Fast Calcium stromen in cardiomyocyten
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Het meten van Fast Calcium stromen in cardiomyocyten

Article DOI: 10.3791/3505-v • 12:11 min • November 29th, 2011
November 29th, 2011 •
1, 2
1Department of Biological Sciences and Geology, Queensborough Community College, 2Department of Physiology & Biophysics, Stony Brook University

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Wij presenteren een methode om snel (microseconde) calcium gebeurtenissen isoleren van trager stromen in levende cellen met behulp van laser scanning confocale microscopie. De methode meet fluorescentie-intensiteit schommelingen van calcium indicatoren door het opnemen van lijn scans van enkele honderden pixels in een cel. Histogram-analyse laat ons toe om te isoleren van de tijdschalen van de verschillende calcium fluxen.

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Celbiologie Calcium fluxen laser scanning microscopie cardiomyocyten tl-indicatoren
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