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Immunology and Infection

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JoVE Journal Immunology and Infection
Efficacité de la production Parvovirus recombinant avec l'aide d'adénovirus dérivés Systems
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Efficacité de la production Parvovirus recombinant avec l'aide d'adénovirus dérivés Systems

Article DOI: 10.3791/3518-v • 13:47 min • April 23rd, 2012
April 23rd, 2012 •
*1,2, *1, 1,2, 1,2, 1,2
1Tumour Virology Division F010, German Cancer Research Center (DKFZ), 2Inserm Unit 701, German Cancer Research Center (DKFZ)
* These authors contributed equally

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Nous décrivons ici un protocole basé uniquement sur l'infection des cellules, ce qui améliore l'efficacité de la production parvovirus recombinant de plus de 100 fois par rapport à d'autres protocoles en cours d'utilisation. Ce protocole basé sur l'utilisation d'un adénovirus 5 nouvelle base auxiliaire contenant l'unité de transcription parvovirus VP (Ad-VP).

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Immunologie Numéro 62 le parvovirus recombinant adénovirus virus de la production pXX6 virus helper de la virologie l'oncologie
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